Figure 6

The effect of the NHEJ inhibitor and HR inhibitor on knock-in efficiencies by different strategies. (A-B) Knock-in efficiencies of mCherry knock-in at Actb locus by four strategies in mouse ES cells (A) and neurons (B) were measured by FACS and compared with the group treated with NHEJ inhibitor (Scr7 or Nu7026), HR inhibitor (caffeine) or both. Results were presented as mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, unpaired Student's t-test. (C) Schematic overview of HMEJ-mediated gene knock-in in different types of cells. ^*, reported in a recent report²⁰.