Figure 1 | Cell Research

Figure 1

From: Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield

Figure 1

Positional cloning of qNPT1. (A) Appearance of a mature plant. RIL52 was selected from a Chunjiang06 (CJ06) × IR66167-27-5-1-6 cross. Scale bar, 20 cm. (B) Panicle morphology. Scale bar, 5 cm. (C-E) Comparison of RIL52 with its parents with respect to (C) grain number, (D) tiller number and (E) culm diameter. Data are shown as the mean ± SEM (n = 30). The presence of the same lowercase letter denotes a non-significant difference between means (P < 0.05). (F) QTL mapping for grain number, tiller number and culm diameter. (G) Positional cloning of qNPT1. The locus was mapped to a ∼ 4.1 Kbp genomic region flanked by P139 and P143. The numbers below the lines indicate the number of recombinants between qNPT1 and an adjacent marker. The candidate gene was predicted to generate two alternative transcripts. The arrowhead indicates the target site designed for CRISPR/Cas9-based genome editing. (H) Sequence variants at the NPT1 locus in both the promoter and coding regions shown in G. The specific nucleotide variants of the npt1 allele are indicated by the pink boxes.

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