Figure 2

Effect of functional OsOTUB1 on plant architecture and grain yield. (A) Mature plant morphology. Scale bar, 20 cm. (B) Loss-of-function mutations of OsOTUB1 generated via CRISPR/Cas9-based genome editing. The target sequence indicated by the green boxes is located on the reverse strand of OsOTUB1.1 shown in Figure 1G. The osotub1-C1 mutant harbors a 7-bp deletion (in red) that results in a premature stop codon. (C) Heading date. (D) Plant height. (E) Diameter of the uppermost internode. (F) Number of tillers per plant. (G) Panicle length. (H) Number of primary branches per panicle. (I) Number of secondary branches per panicle. (J) Number of grains per panicle. (K) 1 000-grain weight. (L) Overall grain yield per plant. Data are shown as the mean ± SEM (n = 288). All phenotypic data were measured in paddy-grown plants under normal cultivation conditions. The presence of the same lowercase letter denotes a non-significant difference between means (P < 0.05).