Figure 4

Phenotypes of transgenic ZH11 plants over-expressing OsOTUB1.1. (A) Two independent transgenic lines showed reduced tiller number and dwarfism. Scale bar, 10 cm. (B) Panicle size was reduced. Scale bar, 5 cm. (C) Leaves suffered from necrosis. Scale bar, 3 mm. (D) Apoptosis was induced in the flag leaves, assayed via Evans Blue staining. Scale bar, 3 mm. (E) Abundance of OsOTUB1.1 transcript in the young panicle. Transcription relative to the level in ZH11 plants (set to one). Data are shown as the mean ± SEM (n = 3). (F) Plant height. (G) Number of tillers per plant. (H) Number of grains per panicle. Data are shown as the mean ± SEM (n = 60). All phenotypic data were measured in paddy-grown rice plants under normal cultivation conditions. The presence of the same lowercase letter denotes a non-significant difference between means (P < 0.05, panels F to H).