Figure 5 | Cell Research

Figure 5

From: Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield

Figure 5

OsOTUB1 displayed cleavage activity for K48- and K63-linked ubiquitin tetramers. Cleavage activity towards K48- and K63-linked ubiquitin tetramers (Tetra-ub) was analysed using OTUB1, His-OsOTUB1.1 or OsOTUB1.2. The inputs (Ub4) and their cleavage products (trimers (Ub3), dimers (Ub2) and monomers (Ub1)) are labelled on the left. The products were visualised via western blotting using an anti-ubiquitin antibody.

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