Figure 6 | Cell Research

Figure 6

From: Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield

Figure 6

The interaction between OsOTUB1 and OsUBC13 proteins regulates plant architecture. (A) Yeast two-hybrid assays. (B) Pull-down assays using recombinant GST-OsOTUB1 and His-OsUBC13. (C) BiFC assays in rice protoplasts. Scale bar, 10 μm. (D) Morphology of transgenic ZH11 plants. Scale bar, 20 cm. (E) Cross-section of the uppermost internodes. Scale bar, 500 μm. (F) Effect of OsUBC13 on panicle branching. Scale bar, 5 cm. (G) Grain size and shape. Scale bar, 2 mm. (H) Abundance of the OsOTUB1 transcript in young panicles relative to the level in ZH11. Data are shown as the mean ± SEM (n = 3). (I) Number of tillers per plant. (J) Number of grains per panicle. (K) 1 000-grain weight. (L) Diameter of the uppermost internode. Data are shown as the mean ± SEM (n = 30). All phenotypic data were measured in paddy-grown rice plants under normal cultivation conditions. The presence of the same lowercase letter denotes a non-significant difference between means (P < 0.05, panels I to L).

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