Figure 7 | Cell Research

Figure 7

From: Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield

Figure 7

The OsOTUB1-OsSPL14 interaction controls plant architecture. (A) BiFC assays. The N-terminus of YFP-tagged OsSPL14, the SBP domain or a deleted version of OsSPL14 was co-transformed into rice protoplasts along with the C-terminus of YFP-tagged OsOTUB1.1. Panels (from left to right), DAPI staining, YFP signal, differential interference contrast image, merged channels. Scale bar, 10 μm. (B) Co-immunoprecipitation of OsOTUB1.1-GFP and OsSPL14. IB, Immunoblot; IP, immunoprecipitation. (C) Plant morphology. Scale bar, 20 cm. (D) Panicle morphology. Scale bar, 5 cm. (E) OsSPL14 transcript abundance. Transcription relative to the level in ZH11 plants was set to one. Data are shown as the mean ± SEM (n = 3). (F) Number of tillers per plant. (G) Number of grains per panicle. (H) Culm diameter. All phenotypic data were measured in field-grown plants under normal cultivation conditions. Data in F-H are shown as the mean ± SEM (n = 120). The presence of the same lowercase letter denotes a non-significant difference between means (P < 0.05).

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