Figure 4

Cav-3 mutants cause the intracellular retention of Cav-3 wild-type form. Cos-7 cells were transiently co-transfected with cDNA encoding GFP-Cav-3 wild type (WT) in combination either with Cav-3 WT or the Cav-3 T78K mutant. Thirty-six hours post-transfection, cells were fixed and stained with antibodies against GFP. When co-transfected with Cav-3 WT or GFP-Cav-3 WT is localized at the plasma membrane, as expected. When co-transfected with Cav-3 T78K, GFP-Cav-3 WT is retained in a perinuclear compartment, suggesting that Cav-3 T78K behaves in a dominant-negative manner. N, nucleus. Final magnification, × 100. Modified and reproduced by Traverso et al.⁴⁶