Figure 1
Dysferlin exon 32 skipping analysis. RT-PCR analysis of the transcript region flanking exon 32 (exons 30–33) in control fibroblasts-derived myoblasts and fibroblasts-derived myoblasts from patient F1-38-1-2. After treatment with H34DYSF2, a shorter transcript fragment, not present in the negative control, was observed at the expected size (215 bp) and indicated the efficient skipping of exon 32. A similar result was observed after treatment with ESE1-AON used as a positive control.
