Figure 1
From: Evidence for anticipation in Beckwith–Wiedemann syndrome

Family pedigree. The degree of methylation of the locus between H19 and ICR1 was investigated by routine MLPA testing and bisulphite subclone sequencing, indicated by the (first ratio/) and (/middle ratio/), respectively. The methylation of the CTCF binding site 6 (CTS6) within the B1 repeat was investigated by bisulphite next generation sequencing, indicated by the (/final ratio) below the pedigree symbols.