Figure 2 | European Journal of Human Genetics

Figure 2

From: Functional analysis of four LDLR 5′UTR and promoter variants in patients with familial hypercholesterolaemia

Figure 2

(a) Measurement of promoter activity using luciferase assay. Fragments containing the wild type and mutant were cloned into the pGL2 promoter, transfected into Huh7 cells and cultured using DMEM medium. Data were obtained in medium supplemented with 10% bovine calf serum. The mutants were compared with the wild type, normalised to the value of 1. These results are the average of 8–12 repeats in 3 independent experiments. Error bars indicate SEM. (b) Measurement of promoter activity using luciferase assay. Data were obtained in medium supplemented with 10% lipid-depleted bovine calf serum.

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