Figure 1
From: A MYLK variant regulates asthmatic inflammation via alterations in mRNA secondary structure
mRNA secondary structure affects MYLK gene translation efficiency. (a) mRNA secondary structures of MFE for wild-type (WT) MLCK gene and its variants (344A, 721T, 1064T and 1287T). (b) Free energy of the top 30 optimal/suboptimal mRNA secondary structures for each MYLK variants. The solid line depicts the mean of 30 values. (c) Landscape of the local accessibility gap (ΔΔG) between the secondary structures of 721T and 721C around the start codon. ‘0’ on x axis indicates the position of the start codon. The local accessibility was calculated using a sliding window of three nucleotides in length and one nucleotide in step. The windows with ΔΔG >0.5 kcal/mol were highlighted in red. (d) Relative mRNA decay curve of MLCK1-GFP (721T or 721C) in HLMVECs after actinomycin D exposure (5 μg/ml). *P<0.05. (e) Expression levels of MLCK1-GFP (721T or 721C) protein in human lung ECs 48 h after transfection.
