Figure 2
From: A MYLK variant regulates asthmatic inflammation via alterations in mRNA secondary structure
Effect of overexpressed human nmMLCK1 variants (147P or 147S) on asthmatic inflammation in a murine model. OVA-challenged nmMLCK−/− mice received nmMLCK1 express transgene to overexpress nmMLCK1 (147P or 147S). (a) Representative nmMLCK1 overexpression in mouse lung tissues after transfection. (b) Quantified relative levels of expressed nmMLCK variants. (c–e) Effect of nmMLCK1 variant overexpression on inflammatory leukocyte infiltration (macrophage, eosinophil and neutrophil). (f) Effects of nmMLCK1 variant overexpression on airway hyper-reactivity reflected by acetylcholine-induced APTI. (g) Effect of nmMLCK1 variant overexpression on BAL protein levels. *P<0.05 between groups of nmMLCK1 147P and 147S. n=5–6.
