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Fas-mediated apoptosis and expression of related genes in human malignant hematopoietic cells
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  • Published: 01 December 2000

Fas-mediated apoptosis and expression of related genes in human malignant hematopoietic cells

  • Kyung-Mi Kim1,
  • Keehyun Lee,
  • Young-Sook Hong &
  • …
  • Hae-Young Park 

Experimental & Molecular Medicine volume 32, pages 246–254 (2000)Cite this article

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Abstract

Fas transduces apoptotic signals upon cross-linking with the Fas ligand (FasL), which is experimentally replaced by agonistic anti-Fas monoclonal antibodies (mAb). Of eight human malignant hematopoietic cell lines (HL-60, KG-1, THP-1, K562, U937, Jurkat, IM-9, RPMI-8226) examined by flow cytometric analysis, all, except K562, were found to be positive for surface Fas antigen. However, despite surface Fas expression, the agonistic anti-Fas mAb (7C11) induced apoptosis in only three of seven Fas-expressing cell lines (KG-1, Jurkat and IM-9). This Fas-resistance did not correlated with high levels of mRNA either for DcR3, a decoy receptor for FasL, or for FAP-1, a Fas-associated phosphatase that can block the apoptotic function of Fas. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis did not show consistent differences in the expression of Bcl-2 and Bax between Fas-sensitive and Fas-resistant cell lines examined. These findings indicated that the presence or absence of mRNA expression of DcR3, FAP-1, Bcl-2 and Bax did not always correlate with relative sensitivity to Fas-mediated apoptosis. Treatment of cells with cycloheximide converted the phenotype of resistant cell lines from Fas-resistant to Fas-sensitive, and enhanced the sensitivity of Fas-sensitive cell lines. These results suggest that the Fas-resistance is dependent on the presence of labile proteins that determine resistance to Fas-mediated apoptosis and the apoptotic machinery is already in place in Fas-resistant cell lines.

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Authors and Affiliations

  1. Department of Biochemistry and Medical Research Center, College of Medicine, Ewha Womans University, Seoul, Korea

    Kyung-Mi Kim

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  1. Kyung-Mi Kim
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  2. Keehyun Lee
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  3. Young-Sook Hong
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  4. Hae-Young Park
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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kim, KM., Lee, K., Hong, YS. et al. Fas-mediated apoptosis and expression of related genes in human malignant hematopoietic cells. Exp Mol Med 32, 246–254 (2000). https://doi.org/10.1038/emm.2000.41

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  • Published: 01 December 2000

  • Issue date: 01 December 2000

  • DOI: https://doi.org/10.1038/emm.2000.41

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Keywords

  • Apoptosis
  • Fas
  • DcR3
  • FAP-1
  • Bcl-2
  • Bax
  • cycloheximide

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