Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Advertisement

Experimental & Molecular Medicine
  • View all journals
  • Search
  • My Account Login
  • Content Explore content
  • About the journal
  • Publish with us
  • Sign up for alerts
  • RSS feed
  1. nature
  2. experimental & molecular medicine
  3. articles
  4. article
Study of ethidium bromide interaction peculiarities with DNA
Download PDF
Download PDF
  • Article
  • Open access
  • Published: 01 December 2001

Study of ethidium bromide interaction peculiarities with DNA

  • P O Vardevanyan1,
  • A P Antonyan,
  • G A Manukyan &
  • …
  • A T Karapetyan 

Experimental & Molecular Medicine volume 33, pages 205–208 (2001)Cite this article

  • 1438 Accesses

  • 15 Citations

  • Metrics details

Abstract

The helix-coil transition of DNA-ethidium bromide complexes in an interval of ionic strength of 2.0 x 10(-3) M ≤ muNa+ ≤ 2.0 x 10(-2) M has been investigated. It has been revealed that at the certain high ligand-DNA ratios (r(b)) the transition interval of the complex-(ΔT) becomes equal to that of DNA itself (ΔTo). It has been shown that the values of r(b) at which δ ΔT=ΔT-ΔT0=0 depends on ionic strength of a solution. Further increasing of ligand concentration leads to its conversion from stabilizer into the destabilizer of the double-stranded DNA.

Similar content being viewed by others

Counterintuitive DNA destabilization by monovalent salt at high concentrations due to overcharging

Article Open access 02 January 2025

Predicting the effect of binding molecules on the shape and mechanical properties of structured DNA assemblies

Article Open access 31 July 2024

Hexaferrocenium tri[hexa(isothiocyanato)iron(III)] trihydroxonium complex as a new DNA intercalator for electrochemical DNA biosensor

Article Open access 12 April 2021

Article PDF

Author information

Authors and Affiliations

  1. Biophysics Department of the Biological Faculty of Yerevan State University, Armenia

    P O Vardevanyan

Authors
  1. P O Vardevanyan
    View author publications

    Search author on:PubMed Google Scholar

  2. A P Antonyan
    View author publications

    Search author on:PubMed Google Scholar

  3. G A Manukyan
    View author publications

    Search author on:PubMed Google Scholar

  4. A T Karapetyan
    View author publications

    Search author on:PubMed Google Scholar

Rights and permissions

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Vardevanyan, P., Antonyan, A., Manukyan, G. et al. Study of ethidium bromide interaction peculiarities with DNA. Exp Mol Med 33, 205–208 (2001). https://doi.org/10.1038/emm.2001.34

Download citation

  • Published: 01 December 2001

  • Issue date: 01 December 2001

  • DOI: https://doi.org/10.1038/emm.2001.34

Share this article

Anyone you share the following link with will be able to read this content:

Sorry, a shareable link is not currently available for this article.

Provided by the Springer Nature SharedIt content-sharing initiative

Keywords

  • DNA
  • ethidium bromide
  • mode of binding
  • bell-shape curve
  • melting curve
Download PDF

Advertisement

Explore content

  • Research articles
  • Reviews & Analysis
  • News & Comment
  • Current issue
  • Collections
  • Sign up for alerts
  • RSS feed

About the journal

  • Special Feature
  • Journal Information
  • About the Editors
  • About the Partner
  • Contact
  • For Advertisers
  • Press Releases
  • Open Access Fees and Funding

Publish with us

  • For Authors & Referees
  • Language editing services
  • Submit manuscript

Search

Advanced search

Quick links

  • Explore articles by subject
  • Find a job
  • Guide to authors
  • Editorial policies

Experimental & Molecular Medicine (Exp Mol Med)

ISSN 2092-6413 (online)

ISSN 1226-3613 (print)

nature.com sitemap

About Nature Portfolio

  • About us
  • Press releases
  • Press office
  • Contact us

Discover content

  • Journals A-Z
  • Articles by subject
  • protocols.io
  • Nature Index

Publishing policies

  • Nature portfolio policies
  • Open access

Author & Researcher services

  • Reprints & permissions
  • Research data
  • Language editing
  • Scientific editing
  • Nature Masterclasses
  • Research Solutions

Libraries & institutions

  • Librarian service & tools
  • Librarian portal
  • Open research
  • Recommend to library

Advertising & partnerships

  • Advertising
  • Partnerships & Services
  • Media kits
  • Branded content

Professional development

  • Nature Awards
  • Nature Careers
  • Nature Conferences

Regional websites

  • Nature Africa
  • Nature China
  • Nature India
  • Nature Japan
  • Nature Middle East
  • Privacy Policy
  • Use of cookies
  • Legal notice
  • Accessibility statement
  • Terms & Conditions
  • Your US state privacy rights
Springer Nature

© 2025 Springer Nature Limited