Abstract
The pH-induced helix-coil transition of DNA and its complexes with EtBr is carried out at acidic pH in a wide interval of change of concentration ratio of EtBr/DNA. The binding isotherms of EtBr on double and single-stranded DNA at pH = 7.0 and pH = 3.0 (t = 25 °C) are obtained by absorption and fluorimetric methods. Binding constants (K) and number of bases (n), corresponding to one binding site were determined. Non fluorescent "strong" complex with ds-DNA at pH = 7.0 and t = 25 °C as well as "strong" and "weak" complexes with ss-DNA at pH = 3.0 and t = 25 °C are revealed.
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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Vardevanyan, P., Antonyan, A., Parsadanyan, M. et al. The binding of ethidium bromide with DNA: interaction with single- and double-stranded structures. Exp Mol Med 35, 527–533 (2003). https://doi.org/10.1038/emm.2003.68
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DOI: https://doi.org/10.1038/emm.2003.68
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