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Coordinated transcriptional regulation of calmegin, a testis-specific molecular chaperon, by histone deacetylase and CpG methyltransferase
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  • Published: 01 October 2005

Coordinated transcriptional regulation of calmegin, a testis-specific molecular chaperon, by histone deacetylase and CpG methyltransferase

  • Dong Hoon Kim1,
  • Joong Sup Shim &
  • Ho Jeong Kwon 

Experimental & Molecular Medicine volume 37, pages 492–496 (2005)Cite this article

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Abstract

Calmegin is a testis-specific molecular chaperon playing a key role in spermatogenesis. However, the transcriptional regulatory mechanisms for calmegin expression are entirely unknown. Herein, we revealed that calmegin is transcriptionally regulated by histone deacetylase (HDAC) and CpG methyltransferase. The cDNA microarray analysis of the human fibrosarcoma cells treated with trichostatin A (TSA) showed an increased level of calmegin mRNA. The induction of calmegin mRNA by TSA was added by the treatment with 5-aza-2'-deoxycytidine (5'Aza- dC), implying that epigenetic alterations are involved in the transcriptional repression of the gene. Moreover, chromatin immunoprecipitation assay using an anti-acetyl-histone H3 antibody exhibited that the proximal region (-152~-31) of the calmegin promoter is responsible for HDAC-mediated transcriptional repression of the gene. These results demonstrate that calmegin expression is regulated by HDAC and CpG methyltransferase in a coordinative way.

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Authors and Affiliations

  1. Chemical Genomics Laboratory, Department of Biotechnology, College of Engineering, Biotechnology Industrialization Institute, Yonsei University, 134 Shinchong-dong, Seodaemun-gu, Seoul, 120-749, Korea

    Dong Hoon Kim

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  1. Dong Hoon Kim
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  2. Joong Sup Shim
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  3. Ho Jeong Kwon
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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kim, D., Shim, J. & Kwon, H. Coordinated transcriptional regulation of calmegin, a testis-specific molecular chaperon, by histone deacetylase and CpG methyltransferase. Exp Mol Med 37, 492–496 (2005). https://doi.org/10.1038/emm.2005.61

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  • Published: 01 October 2005

  • Issue date: 01 October 2005

  • DOI: https://doi.org/10.1038/emm.2005.61

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Keywords

  • calmegin
  • DNA methylation
  • histone deacetylase
  • microarray analysis
  • spermatogenesis

This article is cited by

  • Histone chaperones regulate histone exchange during transcription

    • Hye-Jin Kim
    • Ja-Hwan Seol
    • Eun-Jung Cho

    The EMBO Journal (2007)

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Experimental & Molecular Medicine (Exp Mol Med)

ISSN 2092-6413 (online)

ISSN 1226-3613 (print)

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