Naltrexone influences protein kinase Cε and integrin α7 activity in SH-SY5Y neuroblastoma cells

Abstract

Alcohol influences the neuroadaptation of brain cells where receptors and enzymes like protein kinase C (PKC) exist. Naltrexone acts on opioid receptors. However, other mechanisms of action remain unknown. We prepared SH-SY5Y neuroblastoma cells, and fed them with 150 mM ethanol for 72 h followed by treatment with naltrexone for 24 h. We performed microarray analysis and reverse transcriptase-polymerase chain reaction. Our results showed that PKCε increased 1.90 times and showed an overall decreasing pattern as time increased. Phosphorylated ERK also increased 2.0 times according to the change of PKCε. Integrin α7 increased 2.32 times and showed an increasing pattern as time increased. In conclusion, naltrexone influences PKCε neuronal signaling system and endothelial adhesion molecule integrin α7 in addition to the well-known opioid system.