Figure 2

The sensitivity to doxorubicin and the accumulation of paclitaxel. (A) 1.0 × 10³ cells per well were seeded in 96-well plate to which were added a dilution series of doxorubicin (left panel) and vincristine (right panel) in triplicate and incubated for 4 days under standard culture conditions. Cell viability was assessed using a CellTiter 96 Aqueous One Solution cell proliferation assay kit (Promega). Results are expressed as means ± SD of three independent experiments. T-test: ^*P < 0.05. (B) Control and mtDNA-depleted HCT-8 cells were seeded in 6-well plates and grown for 48 h. The cells were then incubated with 50 nM [³H]-paclitaxel for 2 h at 37℃. At the end of incubation, the cells were cooled on ice, washed three times with ice-cold PBS, and solubilized with 0.2 ml of 1% SDS. The radioactivity in each sample was determined by scintillation counting. The Results are expressed as the means ± SD of three independent experiments. T-test: ^*P < 0.05.