Figure 3
Expression of MDR1 mRNA and P-glycoprotein in control and mtDNA-depleted HCT-8 cells. (A) Cell lysates were prepared from control and mtDNA-depleted HCT-8 cells. Equal amounts (20 µg proteins) of cell lysates were separated via 7% SDS-PAGE and subjected to immunoblotting with anti-P-glycoprotein or anti-actin antibody. A representative result of three independent experiments is shown. (B) Total RNA was isolated from control and mtDNA-depleted HCT-8 cells, and the level of MDR1 transcript was analyzed by real-time PCR. The relative values were expressed as fold increase relative to that observed in control HCT-8 cells. The Results are expressed as the means ± SD of three independent experiments. T-test: *P < 0.01.
