Figure 2
Cellular transduction of Oregon 488-labeled GST:Hoxc81-242 in PPFF cells. PPFF cells were cultured in the presence of Oregon 488-labeled GST:Hoxc81-242 protein (500 nM) as described in Materials and Methods and analyzed under the fluorescence (A) and light microscope (B). Oregon 488 alone (C) and Oregon 488-labeled GST protein (D) were included as controls. (E) Time course of protein (Oregon 488-labeled GST:Hoxc81-242) transduction. After adding the fusion protein GST:Hoxc81-242 labeled with Oregon 488 for 5 min, 10 min, 15 min, 30 min, 1 h, or 2 h, cells were washed with PBS and then fluorescence microscopy was taken.
