Figure 1 | Experimental & Molecular Medicine

Figure 1

From: TNF-α-induced up-regulation of intercellular adhesion molecule-1 is regulated by a Rac-ROS-dependent cascade in human airway epithelial cells

Figure 1

TNF-α induced monocyte adhesion to A549 lung epithelium. A549 cells were grown to confluence in 35-mm plates and stimulated with 10 ng/ml TNF-α or control buffer for 18 h. Then, Calcein-AM-prelabled U937 monocytes were added and we incubated them for additional 15 min. Adhesion of monocytes in response to TNF-α was observed using fluorescence microscopy (A) or counted after the indicated treatment times (B). Cells were counted from six different fields. Results are expressed as means ± SD of three independent experiments.

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