Figure 5
Rac1 is required for TNF-α-induced ICAM-1 expression and adhesiveness. (A) A549 cells and RacN17-transiently-transfected cells were stimulated with 10 ng/ml TNF-α for 12 h. The cell lysates were subjected to immunoblot for detecting the expressions of ICAM-1, Rac1, and HA-RacN17. The relative intensity of ICAM-1 band was measured and expressed as percentages ± SD of three independent experiments. (B) Adhesion assays were conducted with A549 and A549 cells transfected with RacN17 as described in Figure 1 in the presence or absence of 10 ng/ml TNF-α. Data are expressed as means ± SD (n = 30 cells) of three independent experiments.
