Figure 6
Rac1-ROS-linked cascade regulates TNF-α-induced ICAM-1 expression via a stimulation of NF-κB. A549 cells were exposed to TNF-α (10 ng/ml) for 10 min (A) or 12 h (C) in the presence or absence of DPI (A) or PDTC (C) with the indicated concentrations, respectively. Western blotting for IκBα (A) or ICAM-1(C) was carried out to determine the expression levels, respectively. (B) A549 cells were transiently transfected with a reporter gene (pNF-κB-Luc) and DPI was added for 30 min prior to the application of TNF-α (10 ng/ml). The relative luciferase activity was measured. (D) A549 cells were transiently co-transfected with a reporter gene (pNF-κB-Luc) along with pcDNA3 or pcDNA3-RacN17 and the relative luciferase activity was measured using luminometer. These results shown are representative of at least three independent experiments. Statistical significance of NF-κB activation was assessed using unpaired Student's test (*P < 0.05).
