Figure 2 | Experimental & Molecular Medicine

Figure 2

From: T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhances anti-angiogenic and anti-tumor activity via αvβ3 and α5β1 integrins

Figure 2

T-CAM supports adhesion and migration of endothelial cells. (A) The cell adhesion assay was carried out in 96-well plate pre-coated with fastatin, FIII 9-10 and T-CAM (either of these proteins) in dose-dependent manner. The numbers of HUVECs adhering to wells were quantified by enzymatic method as described in "Materials and Methods". (B) HUVECs migration was examined using transwell plates coated with protein in dose-dependent manner. Cells migrated into the lower side of filter were fixed and stained and quantified by counting in different microscopic fields. (C) adhesion of β3 and β5 integrin overexpressing HEK cells to each of these proteins carried out as described above. *P < 0.05; **P < 0.01 versus untreated control.

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