Figure 3 | Experimental & Molecular Medicine

Figure 3

From: T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhances anti-angiogenic and anti-tumor activity via αvβ3 and α5β1 integrins

Figure 3

Identification of integrins mediating adhesion and migration of HUVECs to T-CAM. (A) HUVECs were preincubated with the function-blocking monoclonal antibodies to integrins and then added to 96-well plates precoated with fastatin (a), FIII 9-10 (b) and T-CAM (c). The numbers of attached cells were quantified by enzymatic methods as described above. (B) HUVECs migration were assayed using transwell plates coated with either of these proteins (a, fastatin; b, FIII 9-10; c, T-CAM). Cells were preincubated with function-blocking monoclonal antibody before adding cells into the upper wells of the transwell plates. The number of cells migrated into the lower chamber of filter were quantified after fixing and staining the cells. *P < 0.05; **P < 0.01 versus untreated control.

Back to article page