Figure 3

Effect of siLARS on colony formation and migration of A549 cells. (A) Colony formation assay. A549 cells were transfected with siCon and siLARS, respectively, and incubated in 6-well plates for 6 days. Colonies larger than 1 mm in diameter were counted. Bar chart in the right box represents the percentage of colony numbers in siLARS-treated cells to those in siCon-treated cells. Data represents mean of percentages ± SD of three independent experiments. (B) Soft agar assay. Same cells were incubated in low-melting agarose as described in Materials and Methods. Two weeks later, colonies were photographed and numbers of colonies were counted. (C) Transwell assay. Same cells were incubated in upper chamber of 24-well transwell chambers for migration assay. After 24 h, migrated cells were counted as described in Materials and Methods. The migrated cells were photographed and percentages of migrated siLARStreated cells to migrated siCon-treated cells were calculated. Data represents mean of percentages ± SD of three dependent experiments.