Figure 3
From: Lysophosphatidic acid induces cell migration through the selective activation of Akt1
Akt1 is required for LPA-induced MEF cell migration. (A) Expression of each Akt isoform was ascertained by western blotting with indicated antibodies. (B) LPA-induced migration of established MEF cells was measured. *Significantly different from LPA-stimulated WT cells (P < 0.05). (C) Either Akt1 or Akt2 was re-introduced into Akt1 and Akt2 double deficient (DKO) cells. Ectopic expression of Akt1 or Akt2 was ascertained by western blotting with anti-myc antibody. Comparison of ectopic versus endogenous Akt levels was performed by western blotting with specific antibody against either Akt1 or Akt2. (D and E) LPA- or AOCP-induced migration in DKO cells after re-expression of either Akt1 or Akt2. *Significantly different from LPA- or AOCP-stimulated DKO-Vec cells (P < 0.05). **Values were not statistically different from LPA- or AOCP-stimulated DKO-Vec cells (P > 0.05).
