Figure 4
From: Lysophosphatidic acid induces cell migration through the selective activation of Akt1
Akt1 is selectively activated by LPA stimulation. (A) Cells were either stimulated with LPA (5 µM) for indicated time or stimulated with indicated dose of LPA for 5 min, and LPA-induced phosphorylation at Ser473 of Akt and total Akt level was ascertained by western blotting. (B) Cells were stimulated with either LPA (5 µM) or AOCP (1%) for 5 min and cell extracts were immunoprecipitated with anti-myc antibody. Total Akt and phosphorylated Akt were ascertained by western blotting with anti-myc and anti-phospho-Akt (Ser473) antibodies, respectively.
