Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Spatiotemporal regulation of fibroblast growth factor signal blocking for endoderm formation in Xenopus laevis

Figure 2

(A) Procedure of the bead implantation assay. (B) Bead implanted embryos were analyzed using whole mount in situ hybridization with Darmin probe, in order to label the mid-gut. An FGF-soaked bead implanted embryo showed the repression of Darmin expression around the bead (15/16 affected embryos/total embryos). However, cells neighboring the SU5402 soaked bead showed strong expression of darmin (18/18 affected embryos/total embryos). This shows that the presence or absence of FGF signaling is strongly related to the expression of endodermal genes in embryonic cells during Xenopus embryogenesis (Table S2).

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