Figure 2

The profile of cytokine production (IL-2, IL-4, and IFN-γ) by splenocytes (A, B, and C) and popliteal LN cells (D, E, and F) from animals immunized with rAd expressing glycoproteins (rAd-gB, rAd-gC, and rAd-gD). The responder cells (splenocytes and LN cells from the immunized mice) were mixed with irradiated syngeneic enriched APCs that had been pulsed with UV-inactivated PrV, and incubated for 3 days at 2 weeks after the final immunization. The levels of cytokines in the supernatants of the stimulated T cells were determined by cytokine ELISA. The test was carried out in quadruplicate wells. The bars represent the average ± SD from three independent experiments. P-values in the graphs were calculated using the Student's t-test.