Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Lysophosphatidic acid receptor 2 and Gi/Src pathway mediate cell motility through cyclooxygenase 2 expression in CAOV-3 ovarian cancer cells

Figure 1

LPA induces COX-2 expression through Gi, EGFR and ERK. (A) The cells were serum starved overnight, and stimulated with LPA for indicated times. Cell lysates were analyzed by immunoblotted against anti-COX-1 or anti-COX-2 antibody. As a control, the lysate of NIH-3T3 cells was analyzed in the same gel. (B) The cells were pretreated with or without pharmacological inhibitors of Gi (pertussis toxin), PI3 kinase (LY294002 and wortmannin), EGFR (AG1478), ERK (PD98059 and U0126), followed by stimulation with indicated concentration of LPA. The cell lysates were analyzed by either RT-PCR or immunoblotting. (C) The cells were pretreated with or without indicated pharmacological inhibitors, followed by stimulation with LPA and the cell migration was analyzed at time 0 and 24 h. Results show a representative of three experiments and the mean ± S.D. of three experiments. #P< 0.05 vs. control, *P <0.05 vs. LPA treatment.

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