Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Lysophosphatidic acid receptor 2 and Gi/Src pathway mediate cell motility through cyclooxygenase 2 expression in CAOV-3 ovarian cancer cells

Figure 2

Src is involved in EGFR transactivation. The cells were pretreated with pharmacological inhibitors of Src (PP2) or MMP (GM6001) for 1 h, followed by stimulation with 25 µM LPA. (A) The cell lysates were immunoblotted as described in materials and methods. Anti-p-Tyr antibody was utilized to detect P-EGFR, after the cell lysate was immunoprecipitated with EGFR antibody. Results show a representative blot of three experiments and the mean ± S.D. of three experiments. (B) The cells were transfected with either selective c-Src or negative control siRNA, followed by stimulation with or without 25 µM LPA. (C) Conditioned media was analyzed by gelatin zymography (upper gel) and ELISA (lower graph) to detect MMP-2 activity and total expression, respectively. Results show a representative gel and ELISA of three experiments. #P < 0.05 vs. control, *P < 0.05 vs. LPA treatment.

Back to article page