Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Lysophosphatidic acid receptor 2 and Gi/Src pathway mediate cell motility through cyclooxygenase 2 expression in CAOV-3 ovarian cancer cells

Figure 3

EP2 is involved in LPA-induced cell migration. (A) The cells were pre-incubated with or without pharmacological inhibitors of COX-2 (100 µM NS-398, 50 µM celecoxib), followed by 25 µM LPA stimulation. The cell migration was assessed by wound healing at time 0 h and 24 h. (B) Pre-incubated cells with COX-2 inhibitors were stimulated with LPA, and the amount of PGE2 was assessed by ELISA. (C) The cells were pre-incubated with pharmacological inhibitors of EP2 (AH6809), EP4 (AH23848) or anti-EP2 blocking antibody, followed by LPA stimulation. The cell migration was analyzed by wound healing at time 0h and 24 h. Results show a representative of five experiments and the mean ± S.D. of five experiments. #P < 0.05 vs. control, *P < 0.05 vs. LPA treatment.

Back to article page