Figure 4 | Experimental & Molecular Medicine

Figure 4

From: Lysophosphatidic acid receptor 2 and Gi/Src pathway mediate cell motility through cyclooxygenase 2 expression in CAOV-3 ovarian cancer cells

Figure 4

LPA2 is responsible for phosphorylation of EGFR and ERK, as well as COX-2 expression. (A) Total RNA of CAOV-3 cells were reverse transcribed and amplified utilizing specific primers for each LPA receptors with LPA or not as described in Materials and methods. (B) The cells were pretreated with or without pharmacological inhibitors of LPA1 & LPA3 (VPC32183, Ki16425), followed by stimulation with indicated concentration of LPA. The cell lysates were analyzed by immunoblotting. Results show a representative blot of three experiments. (C) The cells were transfected with either LPA2 specific siRNA or negative control siRNA, followed by stimulation with or without 25 µM LPA. The cell lysates were subject to immunoblotting against each antibody. Results show a representative blot of three experiments.

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