Figure 4 | Experimental & Molecular Medicine

Figure 4

From: CD36 signaling inhibits the translation of heat shock protein 70 induced by oxidized low density lipoprotein through activation of peroxisome proliferators-activated receptor γ

Figure 4

Activation of CD36 or PPARγ inhibits hsp70 protein synthesis in U937 cells. (A) U937 mock-transfectants or dominant negative CD36-transfectants were incubated for 8 h in the absence or presence of OxLDL (80 µg/ml). Cells were collected and analyzed for Hsp70 mRNA expression by Northern blot analysis. GAPDH bands were detected as loading controls. (B-D) U937 cells were pre-treated with heat shock for 20 min at 42℃ and then exposed to 5 µg/ml cycloheximide (CHX) (B), 5 µM troglitazone (Trog) (C), or both reagents (D) after 0, 1, 2, 4, 6 h following heat shock treatment. At 8 h, protein lysates were prepared and subjected to Western blot (WB) analysis to monitor Hsp70 protein levels. (E) U937 cells were transiently transfected with PPARγ or control GAPDH siRNA. After transfection, cells were subjected to heat shock and then exposed to 5 µM troglitazone for 8 h; protein lysates were prepared and subjected to Western blot analysis to monitor Hsp70 protein levels. βf-actin levels served as loading controls. Relative expression levels of Hsp70 are shown as values relative to Hsp70 expression levels of untreated controls (designated as 100%).

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