Figure 1
The morphological and immunohistochemical characteristics of the 4 CC cell lines. (A) Phase contrast images of the 4 CC cell lines that were monolayer-cultured in DMEM supplemented with 10% FBS and 1% antibiotics-antimycotics in a 5% CO2-air conditioned 37℃ incubator. (B) Histochemical findings of the xenotransplanted tumors derived from the 4 CC cell lines (SCK, JCK1, Cho-CK or Choi-CK). The anaplastic spindle cells with a fascicular arrangement in a xenotransplanted tumor derived from SCK cells. The poorly differentiated adenocarcinoma derived from the JCK1 cells. The moderately differentiated and the well differentiated adenocarcinomas derived from the Cho-CK and Choi-CK cells, respectively (H&E, ×100; upper panels). Immunohistochemistry (IHC) with an anti-KRT7 antibody stained for the xenotransplanted tumors derived from the 4 CC cell lines. SCK cell-derived tumor; most of the spindle cells were barely stained. IHC with anti-KRT7 antibody in the xenotransplanted tumor tissues, according to the tumor differentiation (lower panels). (C) Immunohistochemical staining of the original tumor tissues. Vimentin and KRT19 expressions were examined in the sarcomatoid (SCK) and the differentiated (Choi-CK) CC tissues, respectively.
