Figure 3
Genetic alterations of FHIT and Fas/FasL in the CC cells. (A) RT-PCR analysis of the FHIT gene product in the 4 CC cell lines with using nested PCR. The total RNA isolated from the cells was subjected to RT-PCR. Inner primers 5U1 and 3D1 were used (upper). Inner primers MUR5 and RP2 were used (lower). The PCR products were separated on 2% agarose gels. Arrow, the 707 bp or 747 bp FHIT gene product. Lane M, molecular weight markers. (B) Semiquantitative PCR analysis of the Fas/FasL mRNA expression. The total RNA was extracted from the CC cell lines. The Fas/FasL mRNA expression was analyzed by reverse-transcriptase PCR with using primers for Fas/FasL and for β-actin as an internal control, as described in the Methods section.
