Figure 5

Analysis of maturation markers and type of cytokine secretion by DCs. (A) Immature DCs (1 × 10⁵ cells) were cultured for 48 h with various stimuli. For each marker, expression was plotted for % of marker positive cells (bar graph) and the mean fluorescence intensity (MFI, line) obtained in different conditions. (B) Simultaneous quantification of three cytokines (IL-6, IL-10 and IL-12p70) was performed with CBA assay from 48-h supernatants of DCs (5 × 10³ cells). The net concentration is presented here after subtracting the amount secreted by either CXCR3⁺CCR4^-CD4⁺ or CD4⁺ T cells themselves. Samples were in triplicate, and the means ± SD of lymphokine levels are shown. The experiments were conducted at least twice with similar results and a representative set of data is presented. An asterisk indicates statistical significance compared between mDC and CXCR3⁺CD4⁺/DC (^*P = 0.009 and 0.003 for IL-12 and IL-6, respectively) or CD4⁺/DC (^*P = 0.007 for IL-12 and 0.002 for IL-6, respectively).