Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Reactive oxygen species regulate the generation of urokinase plasminogen activator in human hepatoma cells via MAPK pathways after treatment with hepatocyte growth factor

Figure 2

Effects of HGF and H2O2/LY 294002 on Rac-1 activation. Serum-starved cells was pretreated with or without H2O2 (100 µM) for 30 min and then treated with or without 10 ng/ml HGF (A). Serum-starved cells were pretreated with or without LY (10 µM) for 30 min and then treated with or without HGF (B). After incubation for 15 min, cells were collected, washed, and then sonicated. Cell lysates were immunoprecipitated with PAK-1 PBD and Rac1 activation was measured by Western blotting with a Racl antibody. Representative data from 3 independent experiments were shown.

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