Figure 1
From: Involvement of GADD153 and cardiac ankyrin repeat protein in cardiac ischemia-reperfusion injury
Hypoxia induced apoptosis in cardiac myocytes and the concomitant GADD153 expression and CARP downregulation. (A) Cariomyocytes from neonatal rat were cultured under hypoxic conditions and the apoptotic cells were counted by DAPI staining at the indicated time intervals. The values represent the mean ± SE of three independent experiments that were done in duplicate. (B) Thirty micrograms of the extracted proteins were resolved by 12% SDS-PAGE and the proteins were transferred to the membrane. The blots were probed with polyclonal antibodies against GADD153 and CARP and the blots were then stripped and reprobed with a monoclonal antibody to actin as the loading control. The experiments were done at least thrice, and the result of one representative experiment is shown.
