Figure 2

Induction of GADD153 and apoptosis by cardiac ischemia/reperfusion injury in rats. (A) The myocardial sections were stained with TTC 24 h after acute occlusion of the left anterior descending (LAD) artery (left) (n = 8). The infracted area was not stained by TTC (circled area). Immunohistochemical staining against GADD153 was performed in the same level of the mycocardial section (middle) and immunohistochemical staining against CARP was also done (right). (B) Detection of apoptotic cardiomyocytes in association with the GADD153 expression. Immunohistochemical staining against GADD153 was performed in the myocardium of the sham controls and in the ischemic myocardium after 45 min ischemia following 90 min of reperfusion, respectively. Nuclear red staining indicates the GADD153-positive cells (left upper panels). TUNEL-positive cells were not detected or they were rarely detected in the myocardium of the sham controls (left lower panels). Apoptotic cardiac myocytes with TENEL-positivity in the ischemic area after 45 min ischemia following 90 min of reperfusion (right). Scale bar, 200 µm.