Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Thromboxane A2 modulates migration, proliferation, and differentiation of adipose tissue-derived mesenchymal stem cells

Figure 1

Effects of U44619 and prostanoid metabolites on the migration of hADSCs. (A) The mRNA levels of prostanoid receptors (EP1, EP2, EP3, EP4, DP, FP, IP, TP) in hADSCs were determined by RT-PCR analysis. Representative data from three independent experiments are shown. (B) Serum-free media containing 5 µM U46619, iloprost, PGF2α, PGD2, PGE2, or LTB4 were placed in the lower chambers of the Boyden apparatus, and the numbers of migratory hADSCs were determined after 12 h. (C) Indicated concentrations of U46619 were placed in the lower chambers, and the numbers of migratory hADSCs were determined after 12 h. (D) Serum-free media containing vehicles or 5 µM U46619 were placed in the lower chambers, and the number of migrated hADSCs were determined after the indicated time periods. (E) Serum-free media containing 5 µM U46619, 10 ng/ml PDGF-BB, and/or 10 µM SQ29548 were placed into the lower chambers, and the numbers of migrating hADSCs were determined after 12 h. Data represent mean ± SD (n = 4). *, P < 0.05; **, P < 0.01 versus control.

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