Figure 2
From: Soluble mediators from mesenchymal stem cells suppress T cell proliferation by inducing IL-10
Suppression of T cells by MSCs was not dependent on cell-cell contact but was evoked by soluble factors. 5 × 105 BALB/c splenocytes were stimulated with 5 × 105 γ-irradiated C57BL/6 splenocytes in MLR for 72 h. (A) The MLR took place on the bottom of a transwell, and MSCs were added to the indicated upper wells (splenocytes/MSCs ratio was 20:1). (B) Supernatant from day 3 cultures of BALB/c MSCs was added to the MLR at 0 h. MLR culture supernatant and BALB/c fibroblast culture supernatant were used as controls. MLR culture supernatant was obtained from 2 × 106 BALB/c splenocytes co-cultured with 2 × 106 γ-irradiated C57BL/6 splenocytes in 25 cm2 tissue culture flasks for 14 days. BALB/c fibroblast culture supernatant was collected from primary BALB/c fibroblast cells on a 100 mm plate fully cultured for 3 days. The volume of each culture supernatant added was 50% that of MLR. Results are expressed as mean values (± SD) of cpm obtained in triplicate cultures. **P < 0.001.
