Figure 2

LL-37 specifically inhibits SAA-induced IL-8 production in human neutrophils. Human neutrophils were stimulated at various concentrations of LL-37 for 10 min prior to addition of SAA (2 µM) for 24 h (A). Human neutrophils were stimulated with LPS (100 ng/ml) for 24 h in the absence or presence of LL-37 (10 µM) (B). The data represent the mean ± SE of three independent experiments performed in duplicate (A, B). Human neutrophils, stimulated with LL-37 (10 µM), SAA (2 µM), LL-37 (10 µM) + SAA (2 µM), LPS (100 ng/ml), or LL-37 (10 µM) + LPS (100 ng/ml) for 6 h, were harvested for RNA preparation. A RT-PCR was performed using specific primers for human IL-8 and GAPDH. Next, the PCR products were electrophoresed in 2% agarose gel and stained with ethidium bromide. The data obtained from one representative experiment of experiments performed in quadruplicate is shown (C).