Figure 3

Paraoxonase activity and inflammatory markers in mice sera. (A) Paraoxonase activity. Equally diluted serum (20 µl, 20 mg/ml) was added to 230 µl of the paraoxon-ethyl (Sigma Cat# D-9286) containing solution (90 mM Tris-HCl/3.6 mM NaCl/2 mM CaCl₂, pH 8.5). A PON-1 activity of 1 U/L is defined as 1 mmol of p-nitrophenol formed per minute. The mean values not sharing a common letter are significantly different between groups (P < 0.01). The bottom photo shows the immunodetected band of PON from pooled sera of each group. Lower numbers indicate the relative intensity of the detected band, as compared with the TBS-injected sera. (B) Serum concentration of myeloperoxidase (MPO) and lipid hydroperoxide (LPO) were reduced in the rHDL infused groups. Serum MPO and LPO were determined using an commercially available assay kit as described in text. The bottom photo shows the immunodetected band of MPO from pooled sera of each group, which was equally diluted (5 mg of protein/ml). The lower numbers indicate the relative band intensity (BI), as compared with that of TBS-injected sera. The mean values not sharing a common letter are significantly different between groups (P < 0.05).