Figure 5 | Experimental & Molecular Medicine

Figure 5

From: A reconstituted HDL containing V156K or R173C apoA-I exhibited anti-inflammatory activity in apo-E deficient mice and showed resistance to myeloperoxidase-mediated oxidation

Figure 5

Electrophoretic patterns of apoA-I in the lipid-bound state as a result of MPO-mediated oxidation in vitro. Lane Wt, WT-rHDL; V, V156K-rHDL; R, R173C-rHDL. (A) Electrophoretic patterns of apoA-I in rHDL with or without MPO treatment (12% SDS-PAGE). The fragmented band as indicated by arrowhead approximately 22-23 kDa was found in chlorinated WT-rHDL, indicating that WT-apoA-I is more susceptible to the MPO-mediated oxidation than the mutants. Molecular weight of band was determined from comparison with standard proteins (BioRad, low-range molecular weight markers). (B) Change in particle size after chlorination (8-25% native-PAGGE). The WT-rHDL particle size was reduced from 98-100 Å to 94-96 Å, whereas the particle sizes of V156K-rHDL and R173C-rHDL were unchanged. M, high molecular weight markers (AmershamPharmacia) indicating 122, 98, 81, and 71 Å from top.

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