Figure 1

Estrogen affected SaOS-2 cell proliferation. To determine the effect of estrogen on osteoblastic cell growth in vitro, SaOS-2 cell proliferation was examined using MTT. E2 induced a 2-fold increase in cell proliferation in SaOS-2 cells co-treated with the wild-type human ERα expression vector pSG5HEO. *indicates a significant difference between results with and without E2 or ER (P < 0.05).