Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Transcriptional activation of insulin-like growth factor binding protein 6 by 17β-estradiol in SaOS-2 cells

Figure 3

E2 regulation of IGFBP-6 protein level in SaOS-2 cells. SaOS-2 cells were cotreated for 24 h with E2 (0.01, 0.1, and 1 µM) in cells transfected with pSG5HEO. Western blot analysis was used to examine the protein expression of IGFBP-6. The band intensity was quantified using Fluorchem 2.0 software. The level of the IGFBP-6 protein is presented as a multiple of the GAPDH density. Western blot analysis indicated that the level of the IGFBP-6 protein in SaOS-2 cells treated with E2 (0.01, 0.1, 1 µM) and pSG5HEO was 2.8- to 3.5-fold higher than with E2 or pSG5HEO alone. *indicates a significant difference between results with and without E2 and/or ER (P < 0.05).

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