Figure 5
Identification of ERE-2 in the GFBP-6 gene promoter (EMSA). Nuclear extracts from SaOS-2 cells were incubated with a 32P-labeled probe harboring the ERE-2 (Lane 1, 2) of the IGFBP-6 gene. The labeled mutant-type ERE-2 probe was used as a negative control (Lane 3). A 32P-labeled probe harboring ERE-1 (Lane 1-5) was used as a positive control (Lane 5). Responsive banding (see arrow in Figure 5) of the wild-type ERE-1 binding complex was observed (Lane 5). The retarded band of the wild-type and mutant-type ERE-2 binding complex was not observed when a labeled wild-type and mutant-type ERE-2 probe was incubated with nuclear extracts (Lane 2, 3).
